Hotspot

tRNA-Asn(GUU)-ndhF

SSC · 115720-116798 · 1079 bp

Back to Spathiphyllum

Pi 0.221

MarkerSeek score 78.90

Primer available yes

Label tRNA-Asn(GUU)-ndhF

Feature Metadata

label_name: tRNA-Asn(GUU)-ndhF
feature_type: igs
region: SSC
length_bp: 1079
variable_sites: 162
pi: 0.221
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Asn(GUU)-ndhF_p1	ACCTGGGGCTTTTTACAAGG	TTTCTTTGCCGGTGAAGGAA	50.0	45.0	58.0	57.9	1164-1481 bp	1.0	91.4
tRNA-Asn(GUU)-ndhF_p2	CGGGAGAATTGATGACTGCA	TTTCTTTGCCGGTGAAGGAA	50.0	45.0	58.0	57.9	1237-1554 bp	1.0	91.3
tRNA-Asn(GUU)-ndhF_p3	ACAGGGAACGGGTTAGGTTA	TTTCTTTGCCGGTGAAGGAA	50.0	45.0	58.0	57.9	1050-1367 bp	1.0	91.3
tRNA-Asn(GUU)-ndhF_p4	GCCAGGTATTGACGGTGATT	TTTCTTTGCCGGTGAAGGAA	50.0	45.0	58.0	57.9	1127-1444 bp	1.0	91.2
tRNA-Asn(GUU)-ndhF_p5	TGGAACTTGGGTATGGGTCT	TTTCTTTGCCGGTGAAGGAA	50.0	45.0	57.9	57.9	1477-1794 bp	1.0	91.1