Hotspot

tRNA-Leu(CAA)-ndhB

IRb · 100628-101400 · 773 bp

Back to Spathiphyllum

Pi 0.0319

MarkerSeek score 81.20

Primer available yes

Label tRNA-Leu(CAA)-ndhB

Feature Metadata

label_name: tRNA-Leu(CAA)-ndhB
feature_type: igs
region: IRb
length_bp: 773
variable_sites: 23
pi: 0.0319
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Leu(CAA)-ndhB_p1	GTCTCGCGTGTCTACCATTT	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	781-833 bp	1.0	85.8
tRNA-Leu(CAA)-ndhB_p2	CTCGCGTGTCTACCATTTCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	779-831 bp	1.0	85.8
tRNA-Leu(CAA)-ndhB_p3	GCGTGTCTACCATTTCACCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.2	57.9	776-828 bp	1.0	84.3
tRNA-Leu(CAA)-ndhB_p4	TCTCGCGTGTCTACCATTTC	TGCAATTGCTCAGGATACCC	50.0	50.0	57.7	57.9	780-832 bp	1.0	83.6
tRNA-Leu(CAA)-ndhB_p5	TCGCGTGTCTACCATTTCAC	TGCAATTGCTCAGGATACCC	50.0	50.0	58.3	57.9	778-830 bp	1.0	83.5