Hotspot

rpl22-rps19

LSC · 90045-90152 · 108 bp

Back to Spathiphyllum

Pi 0.103

MarkerSeek score 74.70

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 108
variable_sites: 11
pi: 0.103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	GTCCTCGAGCTCTAGGTCTT	GGGTAGAGCCGGATCTAAGT	55.0	55.0	58.0	58.0	982-998 bp	1.0	81.5
rpl22-rps19_p2	GTCCTCGAGCTCTAGGTCTT	TGGGTAGAGCCGGATCTAAG	55.0	55.0	58.0	58.0	983-999 bp	1.0	81.5
rpl22-rps19_p3	CCTCGTCTTCGACCCTCTAT	GGGTAGAGCCGGATCTAAGT	55.0	55.0	58.0	58.0	1390-1405 bp	1.0	81.3
rpl22-rps19_p4	CCTCGTCTTCGACCCTCTAT	TGGGTAGAGCCGGATCTAAG	55.0	55.0	58.0	58.0	1391-1406 bp	1.0	81.3
rpl22-rps19_p5	CCTCGTCTTCGACCCTCTAT	GTTATCCTGCGCTTGGAAGA	55.0	50.0	58.0	58.0	1253-1268 bp	1.0	81.3