Hotspot

tRNA-Thr(UGU)-tRNA-Leu(UAA)

LSC · 50206-51382 · 1177 bp

Back to Spathiphyllum

Pi 0.037

MarkerSeek score 75.70

Primer available yes

Label tRNA-Thr(UGU)-tRNA-Leu(UAA)

Feature Metadata

label_name: tRNA-Thr(UGU)-tRNA-Leu(UAA)
feature_type: igs
region: LSC
length_bp: 1177
variable_sites: 35
pi: 0.037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Thr(UGU)-tRNA-Leu(UAA)_p1	GGAAAAAGCCAGCTATCGGA	ACCAATTTCGCCATATCCCC	50.0	50.0	58.0	58.0	1097-1276 bp	1.0	86.0
tRNA-Thr(UGU)-tRNA-Leu(UAA)_p2	AGGGAAAAAGCCAGCTATCG	ACCAATTTCGCCATATCCCC	50.0	50.0	58.0	58.0	1099-1278 bp	1.0	86.0
tRNA-Thr(UGU)-tRNA-Leu(UAA)_p3	GGAAAAAGCCAGCTATCGGA	TCCATACCAAGGCTCAATGC	50.0	50.0	58.0	57.9	1136-1315 bp	1.0	85.5
tRNA-Thr(UGU)-tRNA-Leu(UAA)_p4	AGGGAAAAAGCCAGCTATCG	TCCATACCAAGGCTCAATGC	50.0	50.0	58.0	57.9	1138-1317 bp	1.0	85.5
tRNA-Thr(UGU)-tRNA-Leu(UAA)_p5	GGAAAAAGCCAGCTATCGGA	TAGCGTCTACCAATTTCGCC	50.0	50.0	58.0	58.1	1105-1284 bp	1.0	85.5