Hotspot

rps4-tRNA-Thr(UGU)

LSC · 49685-50132 · 448 bp

Back to Spathiphyllum

Pi 0.143
MarkerSeek score 84.00
Primer available yes
Label rps4-tRNA-Thr(UGU)

Feature Metadata

label_name
rps4-tRNA-Thr(UGU)
feature_type
igs
region
LSC
length_bp
448
variable_sites
63
pi
0.143
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-tRNA-Thr(UGU)_p1 AGAGCGCAGTTGGGTTTTAA GATGGTCATCGGTTCGACTC 45.0 55.0 58.0 58.2 594-885 bp 1.0 86.2
rps4-tRNA-Thr(UGU)_p2 AGCGCAGTTGGGTTTTAAGA GATGGTCATCGGTTCGACTC 45.0 55.0 58.0 58.2 592-883 bp 1.0 86.2
rps4-tRNA-Thr(UGU)_p3 AGAGCGCAGTTGGGTTTTAA CTCAGAGGTTAGAGCATCGC 45.0 55.0 58.0 57.9 625-916 bp 1.0 86.1
rps4-tRNA-Thr(UGU)_p4 AGCGCAGTTGGGTTTTAAGA CTCAGAGGTTAGAGCATCGC 45.0 55.0 58.0 57.9 623-914 bp 1.0 86.1
rps4-tRNA-Thr(UGU)_p5 GAGCGCAGTTGGGTTTTAAG CTCAGAGGTTAGAGCATCGC 50.0 55.0 57.7 57.9 624-915 bp 1.0 83.7