Hotspot

tRNA-Ser(GCU)-tRNA-Gly(UCC)

LSC · 9600-10976 · 1377 bp

Back to Spathiphyllum

Pi 0.15

MarkerSeek score 77.80

Primer available yes

Label tRNA-Ser(GCU)-tRNA-Gly(UCC)

Feature Metadata

label_name: tRNA-Ser(GCU)-tRNA-Gly(UCC)
feature_type: igs
region: LSC
length_bp: 1377
variable_sites: 139
pi: 0.15
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ser(GCU)-tRNA-Gly(UCC)_p1	GCTTTAGTCCACTCAGCCAT	AGGACGAATCACACTTTTACCA	50.0	40.9	57.6	57.7	997-1440 bp	1.0	70.0
tRNA-Ser(GCU)-tRNA-Gly(UCC)_p2	GCTTTAGTCCACTCAGCCAT	GGACGAATCACACTTTTACCAC	50.0	45.5	57.6	57.6	996-1439 bp	1.0	69.5
tRNA-Ser(GCU)-tRNA-Gly(UCC)_p3	AGTCCACTCAGCCATCTCTC	AGGACGAATCACACTTTTACCA	55.0	40.9	58.5	57.7	992-1435 bp	1.0	69.1
tRNA-Ser(GCU)-tRNA-Gly(UCC)_p4	AGTCCACTCAGCCATCTCTC	GGACGAATCACACTTTTACCAC	55.0	45.5	58.5	57.6	991-1434 bp	1.0	68.7
tRNA-Ser(GCU)-tRNA-Gly(UCC)_p5	TAGTCCACTCAGCCATCTCT	AGGACGAATCACACTTTTACCA	50.0	40.9	57.2	57.7	993-1436 bp	1.0	66.5