Hotspot

psaC-ndhE

SSC · 123386-124182 · 797 bp

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Pi 0.0321

MarkerSeek score 71.50

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 797
variable_sites: 89
pi: 0.0321
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ATCGAGTACGGGCTTTTGTC	CTGCTGAAGCTGCTATTGGA	50.0	50.0	58.0	58.0	1033-1321 bp	0.941176	88.3
psaC-ndhE_p2	ATCGAGTACGGGCTTTTGTC	GCTGAAGCTGCTATTGGACT	50.0	50.0	58.0	58.0	1031-1319 bp	0.941176	88.3
psaC-ndhE_p3	ATTCCCAGTCTCCAGTGGAA	CTGCTGAAGCTGCTATTGGA	50.0	50.0	58.0	58.0	1334-1622 bp	0.941176	87.3
psaC-ndhE_p4	TCCCAGTCTCCAGTGGAAAT	CTGCTGAAGCTGCTATTGGA	50.0	50.0	58.0	58.0	1332-1620 bp	0.941176	87.3
psaC-ndhE_p5	TCCCAGTCTCCAGTGGAAAT	GCTGAAGCTGCTATTGGACT	50.0	50.0	58.0	58.0	1330-1618 bp	0.941176	87.3