Hotspot

ndhF-rpl32

SSC · 118199-119152 · 954 bp

Back to Sparganium

Pi 0.0269

MarkerSeek score 73.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 954
variable_sites: 101
pi: 0.0269
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGAAAAGGTATTATCCATGCA	TTTGACATAGAAGTACGTTTTT	33.3	27.3	52.6	52.0	1022-1067 bp	1.0	49.5
ndhF-rpl32_p2	AGAAAAGGTATTATCCATGCA	TTTTGACATAGAAGTACGTTTT	33.3	27.3	52.6	52.0	1023-1068 bp	1.0	49.4
ndhF-rpl32_p3	AGAAAAGGTATTATCCATGCA	TTTTTGACATAGAAGTACGTTT	33.3	27.3	52.6	52.0	1024-1069 bp	1.0	49.4
ndhF-rpl32_p4	AGAAAAGGTATTATCCATGCA	TCTTCCAAATATTTCTACGAATACG	33.3	32.0	52.6	55.4	1051-1096 bp	1.0	49.2
ndhF-rpl32_p5	AGAAAAGGTATTATCCATGCAT	TCTTCCAAATATTTCTACGAATACG	31.8	32.0	53.2	55.4	1051-1096 bp	1.0	49.2