Hotspot

rpoA-rps11

LSC · 83778-83845 · 68 bp

Back to Sparganium

Pi 0.0222

MarkerSeek score 63.80

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 68
variable_sites: 11
pi: 0.0222
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	TGCTACTTCCTCTCGAACCA	ACGAGATGCAGCATTACGAG	50.0	50.0	58.1	58.2	197-204 bp	1.0	85.3
rpoA-rps11_p2	TGCTACTTCCTCTCGAACCA	GACGAGATGCAGCATTACGA	50.0	50.0	58.1	58.2	198-205 bp	1.0	85.2
rpoA-rps11_p3	ATTGTTCGAGTGGATCCTGC	ACGAGATGCAGCATTACGAG	50.0	50.0	58.0	58.2	214-221 bp	1.0	85.1
rpoA-rps11_p4	ATTGTTCGAGTGGATCCTGC	GACGAGATGCAGCATTACGA	50.0	50.0	58.0	58.2	215-222 bp	1.0	85.0
rpoA-rps11_p5	TCGAGTGGATCCTGCTACTT	ACGAGATGCAGCATTACGAG	50.0	50.0	57.9	58.2	209-216 bp	1.0	84.3