Hotspot

petD-rpoA

LSC · 82543-82757 · 215 bp

Back to Sparganium

Pi 0.03

MarkerSeek score 60.50

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 215
variable_sites: 22
pi: 0.03
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GTTGTAGTTGCAACTCCGGA	GCGGATATCGTATGGAACCC	50.0	55.0	58.1	58.0	1436-1517 bp	1.0	84.3
petD-rpoA_p2	CTCGAGCCGGATGATGAAAA	GCGGATATCGTATGGAACCC	50.0	55.0	58.1	58.0	1306-1326 bp	0.941176	80.4
petD-rpoA_p3	CTCGAGCCGGATGATGAAAA	GGTCCGAGATATGTAACCGC	50.0	55.0	58.1	57.9	1436-1456 bp	0.941176	79.9
petD-rpoA_p4	CTCGAGCCGGATGATGAAAA	TAGCTAACCTGACGGAACCA	50.0	50.0	58.1	58.1	1363-1383 bp	0.941176	79.9
petD-rpoA_p5	CTCGAGCCGGATGATGAAAA	ACCGTCCGTGGAAATAGTTG	50.0	50.0	58.1	57.9	1398-1418 bp	0.941176	79.7