Hotspot

trnW-CCA-trnP-UGG

LSC · 71105-71255 · 151 bp

Back to Sparganium

Pi 0.0261

MarkerSeek score 60.90

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 151
variable_sites: 18
pi: 0.0261
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GCGTTCTACCGAACTGAACT	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.9	58.1	206-209 bp	1.0	86.4
trnW-CCA-trnP-UGG_p2	GCGTTCTACCGAACTGAACT	CACGGGTTCAAATCCTGTCA	50.0	50.0	57.9	58.1	204-207 bp	1.0	86.4
trnW-CCA-trnP-UGG_p3	GCGTTCTACCGAACTGAACT	TGTCACGGGTTCAAATCCTG	50.0	50.0	57.9	58.1	207-210 bp	1.0	86.3
trnW-CCA-trnP-UGG_p4	GCGTTCTACCGAACTGAACT	GGGTACAAAATGTCACGGGT	50.0	50.0	57.9	58.1	217-220 bp	1.0	86.1
trnW-CCA-trnP-UGG_p5	GCGTTCTACCGAACTGAACT	TGGGTACAAAATGTCACGGG	50.0	50.0	57.9	58.1	218-221 bp	1.0	86.0