Hotspot

petA-psbJ

LSC · 67176-68318 · 1143 bp

Back to Sparganium

Pi 0.0198

MarkerSeek score 58.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1143
variable_sites: 69
pi: 0.0198
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CAGGGCCAGAACTTCTTGTT	TCCCTATTCATTGCGGGTTG	50.0	50.0	58.0	57.9	2052-2077 bp	1.0	84.2
petA-psbJ_p2	CAGGGCCAGAACTTCTTGTT	GGGTTAGCTTACGACGTGTT	50.0	50.0	58.0	57.9	2016-2041 bp	1.0	84.1
petA-psbJ_p3	CAGGGCCAGAACTTCTTGTT	ACGGACTAGCTGTACCTACC	50.0	55.0	58.0	58.0	1816-1841 bp	1.0	84.0
petA-psbJ_p4	CAGGGCCAGAACTTCTTGTT	GGACTAGCTGTACCTACCGT	50.0	55.0	58.0	58.0	1814-1839 bp	1.0	84.0
petA-psbJ_p5	TAGGTGGGAACAGAGGAAGG	GGACTAGCTGTACCTACCGT	55.0	55.0	58.0	58.0	1991-2016 bp	0.941176	81.1