Hotspot

atpB-rbcL

LSC · 57625-58441 · 817 bp

Back to Sparganium

Pi 0.00921

MarkerSeek score 60.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 817
variable_sites: 35
pi: 0.00921
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CTTCAAGTGTGGAAACCCCA	ACACCAGCTTTAAATCCAGCA	50.0	42.9	57.9	58.1	899-931 bp	1.0	75.5
atpB-rbcL_p2	CTTCAAGTGTGGAAACCCCA	CCTTGGTTTCGTACTCAGGAG	50.0	52.4	57.9	58.0	957-978 bp	0.941176	71.5
atpB-rbcL_p3	TGGGCCAATAATTTGAGCGA	CCTTGGTTTCGTACTCAGGAG	45.0	52.4	57.9	58.0	995-1016 bp	0.941176	70.8
atpB-rbcL_p4	CTTCAAGTGTGGAAACCCCA	CCTTGGTTTCGTACTCAGGA	50.0	50.0	57.9	56.9	957-978 bp	0.941176	70.8
atpB-rbcL_p5	CTTCAAGTGTGGAAACCCCA	TCCTTGGTTTCGTACTCAGG	50.0	50.0	57.9	56.9	958-979 bp	0.941176	70.8