Hotspot

atpH-atpI

LSC · 14825-15773 · 949 bp

Back to Sparganium

Pi 0.0211

MarkerSeek score 65.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 949
variable_sites: 63
pi: 0.0211
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.1	57.8	983-1011 bp	1.0	86.8
atpH-atpI_p2	AGCCAATCCAGCAGCAATAA	CGAATCCATGGAGGGTCATC	45.0	55.0	57.9	57.8	1001-1029 bp	1.0	86.3
atpH-atpI_p3	CAGCAGCAATAACAGAAGCG	CGAATCCATGGAGGGTCATC	50.0	55.0	57.8	57.8	993-1021 bp	1.0	86.2
atpH-atpI_p4	GCCAATCCAGCAGCAATAAC	CGAATCCATGGAGGGTCATC	50.0	55.0	57.8	57.8	1000-1028 bp	1.0	85.8
atpH-atpI_p5	GTCCAATAGAAGCAAGCCCT	CGAATCCATGGAGGGTCATC	50.0	55.0	57.6	57.8	1023-1051 bp	1.0	83.9