Hotspot

rps19

LSC · 208-489 · 282 bp

Back to Sorghum

Pi 0

MarkerSeek score 27.50

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 282
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 27.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TGGACATAGGATGCCACTCT	GAAGGAAGGAAAGCCGGAAA	50.0	50.0	57.8	58.1	423 bp	0.166667	44.5
rps19_p2	TGGACATAGGATGCCACTCT	AAGGAAAGCCGGAAATACCC	50.0	50.0	57.8	57.9	418 bp	0.166667	44.1
rps19_p3	TGGACATAGGATGCCACTCT	GGAAAGCCGGAAATACCCAA	50.0	50.0	57.8	58.2	416 bp	0.166667	44.0
rps19_p4	GTGGACATAGGATGCCACTC	GAAGGAAGGAAAGCCGGAAA	55.0	50.0	57.8	58.1	424 bp	0.166667	43.9
rps19_p5	GTGGACATAGGATGCCACTC	AAGGAAAGCCGGAAATACCC	55.0	50.0	57.8	57.9	419 bp	0.166667	43.6