Hotspot

rps15

IRb · 105621-105857 · 237 bp

Back to Sorghum

Pi 0

MarkerSeek score 28.90

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: IRb
length_bp: 237
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	TGCAAATTTTTGTGTGTACGAGA	ACGAGGCTCATCAAAATGCA	34.8	45.0	57.8	58.2	432 bp	1.0	52.7
rps15_p2	TGCAAATTTTTGTGTGTACGAG	ACGAGGCTCATCAAAATGCA	36.4	45.0	56.4	58.2	432 bp	1.0	49.7
rps15_p3	TGCAAATTTTTGTGTGTACGA	ACGAGGCTCATCAAAATGCA	33.3	45.0	55.2	58.2	432 bp	1.0	48.2
rps15_p4	GCAAATTTTTGTGTGTACGAGA	ACGAGGCTCATCAAAATGCA	36.4	45.0	56.1	58.2	431 bp	1.0	47.6
rps15_p5	TTGCAAATTTTTGTGTGTACGAG	ACGAGGCTCATCAAAATGCA	34.8	45.0	57.1	58.2	433 bp	1.0	47.1