Hotspot

rpoC2

LSC · 27778-32340 · 4563 bp

Back to Sorghum

Pi 0.000847

MarkerSeek score 35.30

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4563
variable_sites: 8
pi: 0.000847
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	ACCTTCCAAGTACTTCAAGGAC	TGACCATGAACAGTTGCTCC	45.5	50.0	57.6	58.1	299 bp	1.0	60.9
rpoC2_p2	ACCTTCCAAGTACTTCAAGGAC	AGTTGCTCCTATAGTGGCCA	45.5	50.0	57.6	58.1	288 bp	1.0	60.8
rpoC2_p3	ACCTTCCAAGTACTTCAAGGAC	GACCATGAACAGTTGCTCCT	45.5	50.0	57.6	57.8	298 bp	1.0	60.2
rpoC2_p4	ACCTTCCAAGTACTTCAAGGAC	CAGTTGCTCCTATAGTGGCC	45.5	55.0	57.6	57.8	289 bp	1.0	59.8
rpoC2_p5	CCTTCCAAGTACTTCAAGGACT	TGACCATGAACAGTTGCTCC	45.5	50.0	57.4	58.1	298 bp	1.0	58.7