Hotspot

rpl16

LSC · 80346-81827 · 1482 bp

Back to Sorghum

Pi 0

MarkerSeek score 43.70

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1482
variable_sites: 0
pi: 0
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCAGAAAGAAAAACCAGGGT	AGACAAGAACCTTGTTTTTCA	42.9	33.3	57.3	53.8	1631-1636 bp	1.0	40.2
rpl16_p2	TCCAGAAAGAAAAACCAGGGT	AAGACAAGAACCTTGTTTTTCA	42.9	31.8	57.3	54.6	1632-1637 bp	1.0	40.2
rpl16_p3	TCCAGAAAGAAAAACCAGGGT	AGACAAGAACCTTGTTTTTCAA	42.9	31.8	57.3	54.6	1631-1636 bp	1.0	40.2
rpl16_p4	TCCAGAAAGAAAAACCAGGGTT	AGACAAGAACCTTGTTTTTCA	40.9	33.3	58.0	53.8	1631-1636 bp	1.0	40.2
rpl16_p5	TTCCAGAAAGAAAAACCAGGGT	AGACAAGAACCTTGTTTTTCA	40.9	33.3	58.0	53.8	1632-1637 bp	1.0	40.2