Hotspot

ndhG

SSC · 113853-114383 · 531 bp

Back to Sorghum

Pi 0.000628

MarkerSeek score 42.20

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 1
pi: 0.000628
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TTGTGTTGGCAGTAGATGGG	ACCTTTTTCCTTCCTTGAATCT	50.0	36.4	57.8	55.5	712 bp	1.0	42.6
ndhG_p2	GTGTTGGCAGTAGATGGGTT	ACCTTTTTCCTTCCTTGAATCT	50.0	36.4	57.8	55.5	710 bp	1.0	42.6
ndhG_p3	TTGTGTTGGCAGTAGATGGG	ACCTTTTTCCTTCCTTGAATCTT	50.0	34.8	57.8	56.3	712 bp	1.0	40.6
ndhG_p4	GTGTTGGCAGTAGATGGGTT	ACCTTTTTCCTTCCTTGAATCTT	50.0	34.8	57.8	56.3	710 bp	1.0	40.6
ndhG_p5	TTGTGTTGGCAGTAGATGGG	ACCTTTTTCCTTCCTTGAATC	50.0	38.1	57.8	54.0	712 bp	1.0	40.1