Hotspot

ccsA

SSC · 109852-110817 · 966 bp

Back to Sorghum

Pi 0.00248

MarkerSeek score 38.50

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 4
pi: 0.00248
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CTCAAGCATCTCGGTTCGAA	TTGAGAACCCCTTGAACGTC	50.0	50.0	58.0	57.8	1159 bp	1.0	78.2
ccsA_p2	AAGCATCTCGGTTCGAATCC	TTGAGAACCCCTTGAACGTC	50.0	50.0	58.1	57.8	1156 bp	1.0	77.8
ccsA_p3	CTCAAGCATCTCGGTTCGAA	GAGAACCCCTTGAACGTCTT	50.0	50.0	58.0	57.5	1157 bp	1.0	75.9
ccsA_p4	CTCAAGCATCTCGGTTCGAA	TGAGAACCCCTTGAACGTCT	50.0	50.0	58.0	58.6	1158 bp	1.0	75.6
ccsA_p5	AAGCATCTCGGTTCGAATCC	GAGAACCCCTTGAACGTCTT	50.0	50.0	58.1	57.5	1154 bp	1.0	75.4