Hotspot

SobiCr008

IRa · 126963-128454 · 1492 bp

Back to Sorghum

Pi 0.000223

MarkerSeek score 43.30

Primer available yes

Label SobiCr008

Feature Metadata

label_name: SobiCr008
feature_type: rRNA
region: IRa
length_bp: 1492
variable_sites: 1
pi: 0.000223
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
SobiCr008_p1	CAGACGTAGCTGCCTTCTTT	CGAATCCGCTTTGTCTACGA	50.0	50.0	57.9	58.1	1623 bp	1.0	78.6
SobiCr008_p2	CAGACGTAGCTGCCTTCTTT	TCCGAATCCGCTTTGTCTAC	50.0	50.0	57.9	57.7	1625 bp	1.0	77.0
SobiCr008_p3	CAGACGTAGCTGCCTTCTTT	AAGACAATTCCGAATCCGCT	50.0	45.0	57.9	57.6	1633 bp	1.0	76.1
SobiCr008_p4	CAGACGTAGCTGCCTTCTTT	AGACAATTCCGAATCCGCTT	50.0	45.0	57.9	57.6	1632 bp	1.0	76.1
SobiCr008_p5	CAGACGTAGCTGCCTTCTTT	ATTCCGAATCCGCTTTGTCT	50.0	45.0	57.9	57.6	1627 bp	1.0	76.1