Hotspot

psbM-petN

LSC · 19159-19965 · 807 bp

Back to Sorghum

Pi 0.00198

MarkerSeek score 41.50

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 807
variable_sites: 3
pi: 0.00198
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGTTCATTCTAGTTCCTACTGCC	TAGTAAGTCTCGCTTGGGCT	43.5	50.0	57.9	58.2	957-958 bp	1.0	54.4
psbM-petN_p2	TGTTCATTCTAGTTCCTACTGCC	GTAGTATGGGGGAGGAGTGG	43.5	60.0	57.9	58.3	901-902 bp	1.0	53.4
psbM-petN_p3	TGTTCATTCTAGTTCCTACTGCC	TAGTATGGGGGAGGAGTGGA	43.5	55.0	57.9	58.4	900-901 bp	1.0	52.7
psbM-petN_p4	GTTCATTCTAGTTCCTACTGCCT	TAGTAAGTCTCGCTTGGGCT	43.5	50.0	57.7	58.2	956-957 bp	1.0	52.4
psbM-petN_p5	TGTTCATTCTAGTTCCTACTGCC	CGCTAGTAGTATGGGGGAGG	43.5	60.0	57.9	58.5	906-907 bp	1.0	52.0