Hotspot

trnG-UCC-trnM-CAU

LSC · 14817-16764 · 1948 bp

Back to Sorghum

Pi 0.000992

MarkerSeek score 39.20

Primer available yes

Label trnG-UCC-trnM-CAU

Feature Metadata

label_name: trnG-UCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 1948
variable_sites: 4
pi: 0.000992
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnM-CAU_p1	ACGAATCACACTTTTACCACT	ACCACTAAGTGAAAAGCCCT	38.1	45.0	55.5	56.7	2062-2063 bp	1.0	41.1
trnG-UCC-trnM-CAU_p2	ACGAATCACACTTTTACCACT	TGAACCGATGACTTATGCCT	38.1	45.0	55.5	56.6	2032-2033 bp	1.0	40.9
trnG-UCC-trnM-CAU_p3	ACGAATCACACTTTTACCACT	CCGATGACTTATGCCTTACCA	38.1	47.6	55.5	57.5	2028-2029 bp	1.0	40.2
trnG-UCC-trnM-CAU_p4	ACGAATCACACTTTTACCACT	ACCGATGACTTATGCCTTACC	38.1	47.6	55.5	57.5	2029-2030 bp	1.0	40.2
trnG-UCC-trnM-CAU_p5	GAACGAATCACACTTTTACCACT	ACCACTAAGTGAAAAGCCCT	39.1	45.0	57.3	56.7	2064-2065 bp	1.0	40.2