Hotspot

trnS-GCU-psbD

LSC · 8945-9937 · 993 bp

Back to Sorghum

Pi 0.000537

MarkerSeek score 36.20

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 993
variable_sites: 1
pi: 0.000537
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	ATAAGCCGGACCATCCTACA	45.0	50.0	58.0	57.9	1142 bp	1.0	79.0
trnS-GCU-psbD_p2	ATTAGCAATCCGCCGCTTTA	AAAACGGTCCCTTCGTAACC	45.0	50.0	58.0	58.1	1117 bp	1.0	78.8
trnS-GCU-psbD_p3	ATTAGCAATCCGCCGCTTTA	TAAGCCGGACCATCCTACAA	45.0	50.0	58.0	58.1	1141 bp	1.0	78.7
trnS-GCU-psbD_p4	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	45.0	50.0	58.0	58.2	1138 bp	1.0	78.4
trnS-GCU-psbD_p5	ATTAGCAATCCGCCGCTTTA	AAACAAAACGGTCCCTTCGT	45.0	45.0	58.0	58.2	1121 bp	1.0	77.8