Hotspot

rpl32-trnL-UAG

SSC · 117726-119194 · 1469 bp

Back to Sorbus

Pi 0.00794
MarkerSeek score 58.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1469
variable_sites
77
pi
0.00794
unique_haplotype_count
33
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGCAGCATTGAAAGCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 1032-1654 bp 1.0 86.5
rpl32-trnL-UAG_p2 GGGCAGCATTGAAAGCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 1033-1655 bp 1.0 86.5
rpl32-trnL-UAG_p3 TAGGGCAGCATTGAAAGCTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 1034-1656 bp 1.0 86.1
rpl32-trnL-UAG_p4 TAGGGCAGCATTGAAAGCTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 1035-1657 bp 1.0 86.1
rpl32-trnL-UAG_p5 GGGCAGCATTGAAAGCTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.8 57.9 1042-1664 bp 1.0 85.6