Hotspot

rpl33-rps18

LSC · 71278-71495 · 218 bp

Back to Sorbus

Pi 0.016
MarkerSeek score 59.10
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
218
variable_sites
16
pi
0.016
unique_haplotype_count
20
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 CGACACAATACGCCTAGTCG TCAATTCGATCCCCCGATTG 55.0 50.0 58.5 57.7 381-428 bp 1.0 80.9
rpl33-rps18_p2 GACACAATACGCCTAGTCGA TCAATTCGATCCCCCGATTG 50.0 50.0 57.2 57.7 380-427 bp 1.0 78.5
rpl33-rps18_p3 TCGACACAATACGCCTAGTC TCAATTCGATCCCCCGATTG 50.0 50.0 57.2 57.7 382-429 bp 1.0 78.5
rpl33-rps18_p4 CGACACAATACGCCTAGTCG ATTCGATCCCCCGATTGGAT 55.0 50.0 58.5 58.6 378-425 bp 1.0 77.9
rpl33-rps18_p5 AGAATCGACACAATACGCCT TCAATTCGATCCCCCGATTG 45.0 50.0 57.0 57.7 386-433 bp 1.0 77.5