Hotspot

psaJ-rpl33

LSC · 70621-71076 · 456 bp

Back to Sorbus

Pi 0.00418
MarkerSeek score 43.10
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
456
variable_sites
19
pi
0.00418
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TCCGGATGCGTTGATATTCC CACGATTTCGAACACAACTGG 50.0 47.6 57.8 58.1 555-588 bp 1.0 75.2
psaJ-rpl33_p2 TCCGGATGCGTTGATATTCC CGGACATCTTTACCCTTAGCC 50.0 52.4 57.8 58.2 511-544 bp 1.0 75.1
psaJ-rpl33_p3 TCCGGATGCGTTGATATTCC TGGAAATGCCCATTGATTCCT 50.0 42.9 57.8 57.9 582-615 bp 1.0 74.7
psaJ-rpl33_p4 TCCGGATGCGTTGATATTCC ACGATTTCGAACACAACTGG 50.0 45.0 57.8 56.7 554-587 bp 1.0 73.4
psaJ-rpl33_p5 TCCGGATGCGTTGATATTCC CGATTTCGAACACAACTGGT 50.0 45.0 57.8 56.7 553-586 bp 1.0 73.4