Hotspot

petA-psbJ

LSC · 66292-67275 · 984 bp

Back to Sorbus

Pi 0.00324
MarkerSeek score 54.90
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
984
variable_sites
28
pi
0.00324
unique_haplotype_count
30
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TGCTCTTCTTGGCATCTGTT AGCCGGTATTCTTGTGATCG 45.0 50.0 57.4 57.8 1146-1191 bp 1.0 77.5
petA-psbJ_p2 TTGCTCTTCTTGGCATCTGT AGCCGGTATTCTTGTGATCG 45.0 50.0 57.4 57.8 1147-1192 bp 1.0 77.5
petA-psbJ_p3 CGTGTCCAAGGTCTTTTGCT AGCCGGTATTCTTGTGATCG 50.0 50.0 58.7 57.8 1162-1207 bp 1.0 76.5
petA-psbJ_p4 GGTCTTTTGCTCTTCTTGGC AGCCGGTATTCTTGTGATCG 50.0 50.0 57.3 57.8 1153-1198 bp 1.0 76.3
petA-psbJ_p5 TCCATTACGTGTCCAAGGTC AGCCGGTATTCTTGTGATCG 50.0 50.0 57.2 57.8 1169-1214 bp 1.0 76.0