Hotspot

ndhC-trnV-UAC

LSC · 53981-54659 · 679 bp

Back to Sorbus

Pi 0.026
MarkerSeek score 72.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
679
variable_sites
76
pi
0.026
unique_haplotype_count
40
primer_available
yes
alignment_reliability
0.92
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 693-901 bp 1.0 89.1
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT GCACGTTGGGTCTTTGAAAC 50.0 50.0 57.9 58.2 757-965 bp 1.0 88.8
ndhC-trnV-UAC_p3 GGGGCTAAAACTCCGGAAAT AATGCACGTTGGGTCTTTGA 50.0 45.0 57.9 58.3 760-968 bp 1.0 87.6
ndhC-trnV-UAC_p4 GGGGCTAAAACTCCGGAAAT ATGCACGTTGGGTCTTTGAA 50.0 45.0 57.9 58.3 759-967 bp 1.0 87.6
ndhC-trnV-UAC_p5 CTGGTCCTTTGCTAATCGGG GCACGTTGGGTCTTTGAAAC 55.0 50.0 58.3 58.2 774-982 bp 1.0 87.1