Hotspot

trnT-UGU-trnL-UAA

LSC · 49235-50497 · 1263 bp

Back to Sorbus

Pi 0.00859
MarkerSeek score 59.40
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
1263
variable_sites
88
pi
0.00859
unique_haplotype_count
35
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 990-1335 bp 1.0 88.4
trnT-UGU-trnL-UAA_p2 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 998-1343 bp 1.0 87.8
trnT-UGU-trnL-UAA_p3 GGAATCGAACCGATGACCAT ACCAATTTCGCCATATCCCC 50.0 50.0 57.8 58.0 1022-1367 bp 1.0 87.4
trnT-UGU-trnL-UAA_p4 GGAATCGAACCGATGACCAT TAGCGTCTACCAATTTCGCC 50.0 50.0 57.8 58.1 1030-1375 bp 1.0 86.8
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 1004-1349 bp 1.0 86.4