Hotspot

psbZ-trnG-UCC

LSC · 38747-39153 · 407 bp

Back to Sorbus

Pi 0.00564
MarkerSeek score 54.30
Primer available yes
Label psbZ-trnG-UCC

Feature Metadata

label_name
psbZ-trnG-UCC
feature_type
igs
region
LSC
length_bp
407
variable_sites
26
pi
0.00564
unique_haplotype_count
28
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-UCC_p1 TTTGCTTCTCCTGAGGGTTG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.7 57.8 556-619 bp 1.0 84.0
psbZ-trnG-UCC_p2 GGGTTGGTCGGGTAACAAAA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.3 57.8 542-605 bp 1.0 83.9
psbZ-trnG-UCC_p3 TTCCGGCACATCATTATGGA CGTCTTCTCCTTGGCAAAGA 45.0 50.0 57.0 57.8 512-575 bp 1.0 78.4
psbZ-trnG-UCC_p4 AGGGTTGGTCGGGTAACAAA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 59.2 57.8 543-606 bp 1.0 77.1
psbZ-trnG-UCC_p5 ATTTGCTTCTCCTGAGGGTTG CGTCTTCTCCTTGGCAAAGA 47.6 50.0 58.2 57.8 557-620 bp 1.0 76.6