Hotspot

trnT-GGU-psbD

LSC · 34063-35467 · 1405 bp

Back to Sorbus

Pi 0.0059
MarkerSeek score 62.90
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1405
variable_sites
58
pi
0.0059
unique_haplotype_count
33
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GGCCAGACCAACCTACAAAA 55.0 50.0 58.1 58.0 1550-1672 bp 1.0 86.3
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GGCCAGACCAACCTACAAAA 50.0 50.0 58.1 58.0 1553-1675 bp 1.0 86.1
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG TAGGCCAGACCAACCTACAA 55.0 50.0 58.1 58.0 1552-1674 bp 1.0 86.1
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG ACCTACAAAAACGAAGCGGT 55.0 45.0 58.1 58.0 1540-1662 bp 1.0 86.1
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA TAGGCCAGACCAACCTACAA 50.0 50.0 58.1 58.0 1555-1677 bp 1.0 86.0