Hotspot

psbM-trnD-GUC

LSC · 31502-32733 · 1232 bp

Back to Sorbus

Pi 0.00438
MarkerSeek score 50.70
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1232
variable_sites
37
pi
0.00438
unique_haplotype_count
28
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TCACCTTGGCTGACTGTTTT TTCAATTGGTCAGAGCACCG 45.0 50.0 57.6 58.5 1380-1424 bp 1.0 77.0
psbM-trnD-GUC_p2 AATCACCTTGGCTGACTGTT TTCAATTGGTCAGAGCACCG 45.0 50.0 57.3 58.5 1382-1426 bp 1.0 75.1
psbM-trnD-GUC_p3 ATCACCTTGGCTGACTGTTT TTCAATTGGTCAGAGCACCG 45.0 50.0 57.3 58.5 1381-1425 bp 1.0 75.1
psbM-trnD-GUC_p4 TCACCTTGGCTGACTGTTTT GTTCAATTGGTCAGAGCACC 45.0 50.0 57.6 57.0 1381-1425 bp 1.0 72.8
psbM-trnD-GUC_p5 ATCACCTTGGCTGACTGTTTT TTCAATTGGTCAGAGCACCG 42.9 50.0 58.1 58.5 1381-1425 bp 1.0 72.1