Hotspot

rpoB-trnC-GCA

LSC · 27801-29017 · 1217 bp

Back to Sorbus

Pi 0.00437
MarkerSeek score 54.60
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1217
variable_sites
37
pi
0.00437
unique_haplotype_count
31
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 TAAGCCCTGCTCAATGAACC CCGGATTTGAACTGGGGAAA 50.0 50.0 57.9 58.1 1363-1400 bp 1.0 81.4
rpoB-trnC-GCA_p2 TAAGCCCTGCTCAATGAACC AAAAGGATTTGCAGTCCCCC 50.0 50.0 57.9 58.4 1345-1382 bp 1.0 79.3
rpoB-trnC-GCA_p3 TATTGCCTCATTTCCACCCC CCGGATTTGAACTGGGGAAA 50.0 50.0 57.5 58.1 1297-1334 bp 1.0 79.0
rpoB-trnC-GCA_p4 CTCTGTTAAGCCCTGCTCAA CCGGATTTGAACTGGGGAAA 50.0 50.0 57.5 58.1 1369-1406 bp 1.0 78.6
rpoB-trnC-GCA_p5 GTTCCTCTGTTAAGCCCTGC CCGGATTTGAACTGGGGAAA 55.0 50.0 58.5 58.1 1373-1410 bp 1.0 78.0