Hotspot

atpH-atpI

LSC · 14342-15509 · 1168 bp

Back to Sorbus

Pi 0.00352
MarkerSeek score 61.00
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1168
variable_sites
40
pi
0.00352
unique_haplotype_count
33
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT GCGAATCCATGGAAGGTCAT 45.0 50.0 58.0 58.0 1208-1262 bp 1.0 85.0
atpH-atpI_p2 AATAGAAGCAAGCCCGACAG GCGAATCCATGGAAGGTCAT 50.0 50.0 58.0 58.0 1242-1296 bp 1.0 84.9
atpH-atpI_p3 ATAACGGAAGCGGCAGAAAT TTTTTGCAACCTTAGCTGCG 45.0 45.0 58.0 57.9 1238-1292 bp 1.0 84.0
atpH-atpI_p4 AATAGAAGCAAGCCCGACAG TTTTTGCAACCTTAGCTGCG 50.0 45.0 58.0 57.9 1272-1326 bp 1.0 83.9
atpH-atpI_p5 AATAACGGAAGCGGCAGAAA GCGAATCCATGGAAGGTCAT 45.0 50.0 58.2 58.0 1209-1263 bp 1.0 83.8