Hotspot

rps16-trnQ-UUG

LSC · 6353-7161 · 809 bp

Back to Sorbus

Pi 0.00506
MarkerSeek score 58.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
809
variable_sites
36
pi
0.00506
unique_haplotype_count
26
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 873-912 bp 1.0 83.9
rps16-trnQ-UUG_p2 GCTTTCTGCCACATCGTTTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.6 58.1 850-889 bp 1.0 82.4
rps16-trnQ-UUG_p3 CCACAGCTGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 883-922 bp 1.0 82.2
rps16-trnQ-UUG_p4 TCCACAGCTGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 884-923 bp 1.0 82.1
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC GTGTCGACTAGAAATGGGGC 50.0 55.0 58.2 58.3 935-974 bp 1.0 81.4