Hotspot

trnH-GUG-psbA

LSC · 116-380 · 265 bp

Back to Sorbus

Pi 0.0227
MarkerSeek score 63.00
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
265
variable_sites
29
pi
0.0227
unique_haplotype_count
34
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 342-386 bp 1.0 89.0
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 356-400 bp 1.0 88.8
trnH-GUG-psbA_p3 ATATTATGGGCGAACGACGG CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 353-397 bp 0.923077 85.7
trnH-GUG-psbA_p4 ATATTATGGGCGAACGACGG TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 356-400 bp 0.923077 85.6
trnH-GUG-psbA_p5 ATATTATGGGCGAACGACGG GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 403-447 bp 0.923077 85.5