Hotspot

rpl20-rps12

LSC · 73059-73847 · 789 bp

Back to Sorbaria

Pi 0.0326

MarkerSeek score 74.40

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 789
variable_sites: 38
pi: 0.0326
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TTGTTCTACGTCTTCGAGCT	AAATGTCACGAAATCCCCCG	45.0	50.0	56.6	58.5	877-902 bp	1.0	71.2
rpl20-rps12_p2	TTGTTCTACGTCTTCGAGCT	CAGCGACGAGGAACATGTAC	45.0	55.0	56.6	58.7	840-865 bp	1.0	69.9
rpl20-rps12_p3	TTGTTCTACGTCTTCGAGCT	AGCGACGAGGAACATGTACT	45.0	50.0	56.6	58.8	839-864 bp	1.0	69.2
rpl20-rps12_p4	TTGTTCTACGTCTTCGAGCT	TCAGCGACGAGGAACATGTA	45.0	50.0	56.6	58.8	841-866 bp	1.0	69.1
rpl20-rps12_p5	TTTGTTCTACGTCTTCGAGCT	AAATGTCACGAAATCCCCCG	42.9	50.0	57.4	58.5	878-903 bp	1.0	69.0