Hotspot

trnV-UAC-trnM-CAU

LSC · 55736-55902 · 167 bp

Back to Sorbaria

Pi 0.0519

MarkerSeek score 77.20

Primer available yes

Label trnV-UAC-trnM-CAU

Feature Metadata

label_name: trnV-UAC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 167
variable_sites: 13
pi: 0.0519
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-trnM-CAU_p1	CCTCTGAAAAACATTGGCGC	CCCGCCGTATGAAAGCAATA	50.0	50.0	58.3	58.4	272 bp	1.0	78.1
trnV-UAC-trnM-CAU_p2	CCTCTGAAAAACATTGGCGC	CCGCCGTATGAAAGCAATAC	50.0	50.0	58.3	57.3	271 bp	1.0	76.1
trnV-UAC-trnM-CAU_p3	CGTGTAAACGAGGTGCTCTA	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.4	58.4	251 bp	1.0	75.9
trnV-UAC-trnM-CAU_p4	CCTCTGAAAAACATTGGCGC	TTTGAACCAATGACTCCCGC	50.0	50.0	58.3	58.8	287 bp	1.0	75.2
trnV-UAC-trnM-CAU_p5	CTCTGAAAAACATTGGCGCG	CCCGCCGTATGAAAGCAATA	50.0	50.0	58.7	58.4	271 bp	1.0	75.2