Hotspot

rps4-trnT-UGU

LSC · 49343-49827 · 485 bp

Back to Sorbaria

Pi 0.0306

MarkerSeek score 78.60

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 485
variable_sites: 17
pi: 0.0306
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TTTCTAAGATCGCTTCCGGC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	575-659 bp	1.0	84.6
rps4-trnT-UGU_p2	TTTCTAAGATCGCTTCCGGC	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.1	57.8	543-627 bp	1.0	83.8
rps4-trnT-UGU_p3	CTAGTTAGCCCCGGTAAAGC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	57.8	57.9	542-626 bp	1.0	83.3
rps4-trnT-UGU_p4	CTAGTTAGCCCCGGTAAAGC	ATGGTCATCGGTTCGATTCC	55.0	50.0	57.8	57.8	510-594 bp	1.0	82.6
rps4-trnT-UGU_p5	GGGGCGAGATTGGTTTCTAA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.6	57.9	588-672 bp	1.0	81.8