Hotspot

rps16-trnQ-UUG

LSC · 6802-7638 · 837 bp

Back to Sorbaria

Pi 0.0715

MarkerSeek score 85.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 837
variable_sites: 89
pi: 0.0715
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CAGCCGATCATGTCCTTCAA	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	1536-1577 bp	1.0	89.2
rps16-trnQ-UUG_p2	GCCGATCATGTCCTTCAAGT	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	1534-1575 bp	1.0	89.2
rps16-trnQ-UUG_p3	CCCTTTTGCCTGTCTCCTTT	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	2075-2096 bp	0.666667	66.6
rps16-trnQ-UUG_p4	CCCTTTTGCCTGTCTCCTTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1467-1474 bp	0.666667	66.4
rps16-trnQ-UUG_p5	TTCTACTCGAGCTCCATCGT	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	1675-1696 bp	0.666667	66.3