Hotspot

psbA-trnK-UUU

LSC · 1733-1979 · 247 bp

Back to Sorbaria

Pi 0.0488

MarkerSeek score 75.70

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 247
variable_sites: 18
pi: 0.0488
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TCCAGTTACAAAAGCGACCC	ACTCCATCCGACTAGTTCCG	50.0	55.0	58.1	58.6	349-416 bp	1.0	79.6
psbA-trnK-UUU_p2	TCCAGTTACAAAAGCGACCC	ACTAGTTCCGGGTTCGAGTC	50.0	55.0	58.1	58.8	339-406 bp	1.0	78.0
psbA-trnK-UUU_p3	TCCAGTTACAAAAGCGACCC	GACTAGTTCCGGGTTCGAGT	50.0	55.0	58.1	58.8	340-407 bp	1.0	78.0
psbA-trnK-UUU_p4	ACGGTTTTCAGTGCTGGTTA	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.7	58.6	369-436 bp	1.0	77.5
psbA-trnK-UUU_p5	TCCAGTTACAAAAGCGACCC	ATCCGACTAGTTCCGGGTTC	50.0	55.0	58.1	58.9	344-411 bp	1.0	77.4