Hotspot

ccsA-ndhD

SSC · 115477-115733 · 257 bp

Back to Sophora

Pi 0.0516

MarkerSeek score 74.40

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 257
variable_sites: 37
pi: 0.0516
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCTGCTCTGGGCTTTCTTAT	TTCTCGATGGCTTCTCTTGC	50.0	50.0	58.0	58.0	710-798 bp	1.0	89.3
ccsA-ndhD_p2	GCTGCTCTGGGCTTTCTTAT	CTCGATGGCTTCTCTTGCAT	50.0	50.0	58.0	58.0	708-796 bp	1.0	89.1
ccsA-ndhD_p3	GCTGCTCTGGGCTTTCTTAT	CTCGACGAAATGGGTGGAAT	50.0	50.0	58.0	58.0	770-858 bp	1.0	88.6
ccsA-ndhD_p4	GCTGCTCTGGGCTTTCTTAT	CGAAATGGGTGGAATGGCTA	50.0	50.0	58.0	57.9	765-853 bp	1.0	88.5
ccsA-ndhD_p5	GCTAATGAGGCATGGGGATC	TTCTCGATGGCTTCTCTTGC	55.0	50.0	58.2	58.0	845-933 bp	1.0	87.3