Hotspot

rps3-rps19

LSC · 84146-84743 · 598 bp

Back to Sophora

Pi 0.0465

MarkerSeek score 78.30

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 598
variable_sites: 77
pi: 0.0465
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	CGCCCTGCAATTTGTACTTG	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.0	58.1	1515-1587 bp	1.0	89.3
rps3-rps19_p2	CGCCCTGCAATTTGTACTTG	CGGAAGGGGTGGACAATTAG	50.0	55.0	58.0	58.0	1784-1856 bp	1.0	88.7
rps3-rps19_p3	CGCCCTGCAATTTGTACTTG	TCGTCGCCGTAGTAAGTAGT	50.0	50.0	58.0	58.0	1415-1487 bp	0.888889	85.1
rps3-rps19_p4	TTATCCGTGCAATCCCTTCG	TCGTCGCCGTAGTAAGTAGT	50.0	50.0	58.1	58.0	1105-1152 bp	0.666667	73.8
rps3-rps19_p5	TTATCCGTGCAATCCCTTCG	CGGAAGGGGTGGACAATTAG	50.0	55.0	58.1	58.0	1474-1521 bp	0.666667	72.6