Hotspot

psaJ-rpl33

LSC · 67128-67563 · 436 bp

Back to Sophora

Pi 0.0464

MarkerSeek score 79.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 436
variable_sites: 60
pi: 0.0464
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	AAAACAAACGCGCTACCAAG	TTGCAACAACAGGGACAGAA	45.0	45.0	57.9	57.9	1296-1350 bp	1.0	90.0
psaJ-rpl33_p2	AAAACAAACGCGCTACCAAG	TTTGCAACAACAGGGACAGA	45.0	45.0	57.9	57.9	1297-1351 bp	1.0	90.0
psaJ-rpl33_p3	AAAACAAACGCGCTACCAAG	AGGGGAAGGGTAACACAGAA	45.0	50.0	57.9	57.9	1361-1415 bp	0.888889	84.1
psaJ-rpl33_p4	TGAACCCGCGACATTTTGTA	AGGGGAAGGGTAACACAGAA	45.0	50.0	58.1	57.9	1384-1438 bp	0.888889	84.0
psaJ-rpl33_p5	TTTGAACCCGCGACATTTTG	AGGGGAAGGGTAACACAGAA	45.0	50.0	57.9	57.9	1386-1440 bp	0.888889	83.9