Hotspot

ycf3-trnS-GGA

LSC · 53775-54044 · 270 bp

Back to Sophora

Pi 0.0405

MarkerSeek score 82.30

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 270
variable_sites: 37
pi: 0.0405
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	45.0	45.0	56.4	57.8	343-413 bp	1.0	77.4
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	TTCCAATGCTACGCCTTGAA	45.0	45.0	56.4	57.8	344-414 bp	1.0	77.4
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	394-464 bp	1.0	76.9
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	395-465 bp	1.0	76.9
ycf3-trnS-GGA_p5	TCTCCTGAAGTTGTCGGAAT	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.2	58.2	393-463 bp	1.0	75.0