Hotspot

trnS-UGA-psbZ

LSC · 44735-45104 · 370 bp

Back to Sophora

Pi 0.0573

MarkerSeek score 79.50

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 370
variable_sites: 55
pi: 0.0573
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	GGGTTCAAAATCAAAGCGGG	55.0	50.0	58.0	57.9	1090-1246 bp	0.888889	86.6
trnS-UGA-psbZ_p2	GAGCTATCAACCACTCAGCC	GGGTTCAAAATCAAAGCGGG	55.0	50.0	58.1	57.9	1097-1253 bp	0.888889	86.3
trnS-UGA-psbZ_p3	GGAGCTATCAACCACTCAGC	GGGTTCAAAATCAAAGCGGG	55.0	50.0	58.1	57.9	1098-1254 bp	0.888889	86.3
trnS-UGA-psbZ_p4	CGTCGACCAAAAGGAGAGAG	GGGTTCAAAATCAAAGCGGG	55.0	50.0	58.0	57.9	1173-1329 bp	0.888889	86.2
trnS-UGA-psbZ_p5	CGTCGTTCCGCGGAATATAT	GGGTTCAAAATCAAAGCGGG	50.0	50.0	58.0	57.9	1629-1785 bp	0.888889	85.0