Hotspot

trnT-GGU-psbD

LSC · 40548-41994 · 1447 bp

Back to Sophora

Pi 0.033

MarkerSeek score 78.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1447
variable_sites: 127
pi: 0.033
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	AATTTACCAAGAGCTATAGTCA	50.0	31.8	58.3	52.3	1447-1584 bp	1.0	50.8
trnT-GGU-psbD_p2	GGCGTAAGTCATCGGTTCAA	AACCAGTCATCCATAATATCAA	50.0	31.8	58.3	52.6	1492-1629 bp	1.0	50.6
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	ACCAGTCATCCATAATATCAAA	50.0	31.8	58.3	52.6	1491-1628 bp	1.0	50.6
trnT-GGU-psbD_p4	GGCGTAAGTCATCGGTTCAA	AACCAGTCATCCATAATATCAAA	50.0	30.4	58.3	53.5	1492-1629 bp	1.0	50.6
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	ACCAGTCATCCATAATATCAAAT	50.0	30.4	58.3	53.3	1491-1628 bp	1.0	50.6