Hotspot

petN-psbM

LSC · 37359-38503 · 1145 bp

Back to Sophora

Pi 0.0508

MarkerSeek score 78.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1145
variable_sites: 140
pi: 0.0508
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GGGATAGCTCAGCTGGTAGA	TCGGTTTCGCTAAAGTGCAT	55.0	45.0	58.0	58.2	2054-2480 bp	1.0	89.5
petN-psbM_p2	TCTTGCTTGGGCTGCTTTAA	ATTCCTTACGGGGATCCCAT	45.0	50.0	58.0	57.9	1420-1858 bp	0.888889	85.4
petN-psbM_p3	GGGATAGCTCAGCTGGTAGA	ATTCCTTACGGGGATCCCAT	55.0	50.0	58.0	57.9	1744-2191 bp	0.888889	84.8
petN-psbM_p4	CCTCGTGTCACCAGTTCAAA	ATTCCTTACGGGGATCCCAT	50.0	50.0	58.1	57.9	1708-2155 bp	0.888889	84.5
petN-psbM_p5	GGGATAGCTCAGCTGGTAGA	CCCGTCAGTCCCGATAGATA	55.0	55.0	58.0	57.8	1833-2160 bp	0.666667	73.9