Hotspot

ndhJ-trnC-GCA

LSC · 12545-13718 · 1174 bp

Back to Sophora

Pi 0.038
MarkerSeek score 80.10
Primer available yes
Label ndhJ-trnC-GCA

Feature Metadata

label_name
ndhJ-trnC-GCA
feature_type
igs
region
LSC
length_bp
1174
variable_sites
128
pi
0.038
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhJ-trnC-GCA_p1 TAGCACCAGGGGGAATGTTA CCGATTAGATCCGATCCGTG 50.0 55.0 58.0 57.7 1892-1963 bp 0.888889 82.0
ndhJ-trnC-GCA_p2 AAATGCAGGGTCGTTTGTCT CCGATTAGATCCGATCCGTG 45.0 55.0 58.0 57.7 2069-2140 bp 0.888889 81.5
ndhJ-trnC-GCA_p3 TAGCACCAGGGGGAATGTTA CGATTAGATCCGATCCGTGG 50.0 55.0 58.0 57.7 1891-1921 bp 0.444444 60.6
ndhJ-trnC-GCA_p4 AAATGCAGGGTCGTTTGTCT CGATTAGATCCGATCCGTGG 45.0 55.0 58.0 57.7 2068-2098 bp 0.444444 60.3
ndhJ-trnC-GCA_p5 TTCCCAATGTGCCTATGACG CGATTAGATCCGATCCGTGG 50.0 55.0 58.0 57.7 1911-1914 bp 0.333333 51.3